Linking integrin conformation to function

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Linking integrin conformation to function.

Integrins are alphabeta heterodimeric adhesion receptors that relay signals bidirectionally across the plasma membrane between the extracellular matrix and cell-surface ligands, and cytoskeletal and signalling effectors. The physical and chemical signals that are controlled by integrins are essential for intercellular communication and underpin all aspects of metazoan existence. To mediate such...

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Relating conformation to function in integrin α5β1.

Whether β1 integrin ectodomains visit conformational states similarly to β2 and β3 integrins has not been characterized. Furthermore, despite a wealth of activating and inhibitory antibodies to β1 integrins, the conformational states that these antibodies stabilize, and the relation of these conformations to function, remain incompletely characterized. Using negative-stain electron microscopy, ...

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Integrin-Mediated Survival Signals Regulate the Apoptotic Function of Bax through Its Conformation and Subcellular Localization

Most normal cells require adhesion to extracellular matrix for survival, but the molecular mechanisms that link cell surface adhesion events to the intracellular apoptotic machinery are not understood. Bcl-2 family proteins regulate apoptosis induced by a variety of cellular insults through acting on internal membranes. A pro-apoptotic Bcl-2 family protein, Bax, is largely present in the cytoso...

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Regulation of α5β1 integrin conformation and function by urokinase receptor binding

Urokinase-type plasminogen activator receptors (uPARs), up-regulated during tumor progression, associate with beta1 integrins, localizing urokinase to sites of cell attachment. Binding of uPAR to the beta-propeller of alpha3beta1 empowers vitronectin adhesion by this integrin. How uPAR modifies other beta1 integrins remains unknown. Using recombinant proteins, we found uPAR directly binds alpha...

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Mouse embryonic fibroblasts isolated from females lacking Xist intron 1 display normal Xist RNA coating. Image courtesy of K. Plath. Where Do Phenotypes eXIST? Xist encodes a lncRNA that is responsible for mediating X chromosome inactivation (XCI) to achieve dosage compensation in mammalian females. Previous work has indicated that binding of pluripotency factors, such as Oct4, Sox2, and Nanog,...

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ژورنال

عنوان ژورنال: Journal of Cell Science

سال: 2009

ISSN: 1477-9137,0021-9533

DOI: 10.1242/jcs.018556